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primary uman lung fibroblasts hlf  (ATCC)


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    ATCC primary uman lung fibroblasts hlf
    Primary Uman Lung Fibroblasts Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/Primary+Lung+Fibroblast%2C+Normal%2C+Human/pm42106936-117-19-25
    Average 99 stars, based on 860 article reviews
    primary uman lung fibroblasts hlf - by Bioz Stars, 2026-10
    99/100 stars

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    Recombinant:

    Article Title: Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers.
    Article Snippet: The culture media was prepared using glucose, peptone, yeast extract purchased from Condalab (Spain), Na2HPO4·12H2O, and citrate acid monohydrate from Sigma-Aldrich (Merck, Spain). .. Human bone marrow-derived mesenchymal stromal cells (hBMMSCs) and human lung fibroblasts (HLFs) were purchased from ATCC (PCS-500-012) as well as the culture media containing 125 pg/mL recombinant human fibroblast growth factor (rh FGF), 15 ng/ mL recombinant human insulin-like growth factor-1, 7% fetal bovine serum (FBS), and 2.4 mM L-alanyl-L-glutamine. .. Human lung fibroblasts (HLFs) were purchased from ATCC (PCS-201-013) as well as the culture media containing 7.5 mM L-glutamine, 1 μg/mL hydrocortisone hemisuccinate, 50 μg/mL ascorbic acid, 5 ng/mL rh FGF, 5 μg/mL recombinant human insulin, and 2% FBS.

    Transformation Assay:

    Article Title: Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entry.
    Article Snippet: .. Human MCF-10A, human retinal pigment epithelial (RPE-1), human lung fibroblasts (HLFs) and transformed human embryonic kidney (HEK293T) cells were obtained from ATCC. ..

    Article Title: Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entry
    Article Snippet: .. Human MCF-10A, human retinal pigment epithelial (RPE-1), human lung fibroblasts (HLFs) and transformed human embryonic kidney (HEK293T) cells were obtained from ATCC. ..

    Multiple Displacement Amplification:

    Article Title: Cyclometalated iridium(III) tetrazine complexes for mitochondria-targeted two-photon photodynamic therapy.
    Article Snippet: The fast-moving field of photodynamic therapy (PDT) has provided fresh opportunities to expand the potential of metallodrugs to combat cancers in a light-controlled manner.. As such, in the present study, a series of cyclometalated Ir(III) complexes modified with a tetrazine functional group (namely, Ir-ppy-Tz, Ir-pbt-Tz, and Ir-dfppy-Tz) are developed as potential two-photon photodynamic anticancer agents.. These complexes target mitochondria but exhibit low toxicity towards HLF primary lung fibroblast normal cells in the dark.



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    primary uman lung fibroblasts hlf  (ATCC)
    99
    ATCC primary uman lung fibroblasts hlf
    Primary Uman Lung Fibroblasts Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/Primary+Lung+Fibroblast%2C+Normal%2C+Human/pm42106936-117-19-25
    Average 99 stars, based on 1 article reviews
    primary uman lung fibroblasts hlf - by Bioz Stars, 2026-10
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    primary human lung fibroblasts hlfs  (ATCC)
    99
    ATCC primary human lung fibroblasts hlfs
    A Human lung smooth muscle cells (HLSMCs), lung <t>fibroblasts</t> <t>(HLFs),</t> and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.
    Primary Human Lung Fibroblasts Hlfs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/Primary+Lung+Fibroblast%2C+Normal%2C+Human/pmc13103083-239-0-17
    Average 99 stars, based on 1 article reviews
    primary human lung fibroblasts hlfs - by Bioz Stars, 2026-10
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    human lung fibroblast cells hlf  (ATCC)
    97
    ATCC human lung fibroblast cells hlf
    A Human lung smooth muscle cells (HLSMCs), lung <t>fibroblasts</t> <t>(HLFs),</t> and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.
    Human Lung Fibroblast Cells Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/MRC-5%3B+Lung+Fibroblast%3B+Human/pmc12639372-37-0-9
    Average 97 stars, based on 1 article reviews
    human lung fibroblast cells hlf - by Bioz Stars, 2026-10
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    human lung fibroblast cell line hlf  (ATCC)
    97
    ATCC human lung fibroblast cell line hlf
    A Human lung smooth muscle cells (HLSMCs), lung <t>fibroblasts</t> <t>(HLFs),</t> and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.
    Human Lung Fibroblast Cell Line Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/MRC-5%3B+Lung+Fibroblast%3B+Human/pmc12580640-34-0-20
    Average 97 stars, based on 1 article reviews
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    normal human lung fibroblasts hlf  (ATCC)
    99
    ATCC normal human lung fibroblasts hlf
    A Human lung smooth muscle cells (HLSMCs), lung <t>fibroblasts</t> <t>(HLFs),</t> and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.
    Normal Human Lung Fibroblasts Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/Primary+Lung+Fibroblast%2C+Normal%2C+Human/pm40897735-66-0-6
    Average 99 stars, based on 1 article reviews
    normal human lung fibroblasts hlf - by Bioz Stars, 2026-10
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    human lung fibroblasts hlfs  (ATCC)
    97
    ATCC human lung fibroblasts hlfs
    A Human lung smooth muscle cells (HLSMCs), lung <t>fibroblasts</t> <t>(HLFs),</t> and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.
    Human Lung Fibroblasts Hlfs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+fibroblasts+hlfs/Primary+Lung+Fibroblast%3B+Fibrosis/pmc12488482-214-7-21
    Average 97 stars, based on 1 article reviews
    human lung fibroblasts hlfs - by Bioz Stars, 2026-10
    97/100 stars
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    Image Search Results


    A Human lung smooth muscle cells (HLSMCs), lung fibroblasts (HLFs), and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.

    Journal: Cell Death Discovery

    Article Title: Trifluoperazine causes mast cell apoptosis through a secretory granule-mediated pathway

    doi: 10.1038/s41420-026-03122-x

    Figure Lengend Snippet: A Human lung smooth muscle cells (HLSMCs), lung fibroblasts (HLFs), and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.

    Article Snippet: Primary human lung fibroblasts (HLFs) (PCS-201-013) and primary human lung smooth muscle cells (HLSMCs)(PCS-130-010) were obtained from American Type Culture Collection (ATCC, Manassas, VA) and cultured following the manufacturer’s instructions.

    Techniques: Staining, Derivative Assay, Control, Agarose Gel Electrophoresis, Marker